Composite

Part:BBa_K553005

Designed by: Giulio Bernardinelli, Veronica Parisi, Francesca Cesaratto   Group: iGEM11_UNITS_Trieste   (2011-09-17)
Revision as of 23:17, 21 September 2011 by Giulio (Talk | contribs)

C. firmi beta-glucosidase PTraI regulated

BBa_K392008 Beta-glucosidase expression under PTraI promoter (BBa_K553002).

The promoter is actively regulated by the TraR - OC8 HLA complex.

https://static.igem.org/mediawiki/parts/3/35/PromTraI_gluco_K553005.pdf

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 105
    Illegal XhoI site found at 1251
    Illegal XhoI site found at 1484
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 740
    Illegal AgeI site found at 183
    Illegal AgeI site found at 242
    Illegal AgeI site found at 636
    Illegal AgeI site found at 831
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI.rc site found at 613


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