Part:BBa_B0034:Experience

This experience page is provided so that any user may enter their experience using this part.
Please enter how you used this part and how it worked out.

User Reviews

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••••• Antiquity

This review comes from the old result system and indicates that this part worked in some test.

••••• Aberdeen_Scotland 2009

Our miniprep, double digest and gel worked properly. We used this part in fusion PCR for building our biobrick BBa_K182102. The fusion PCR, cloning and sequence were all correct.

••••• iGEM Groningen 2009

The ligation of part BBa_K190028 behind the RBS was successful, confirmed by gel (correct vector size after digestion with EcoRI and PstI) and sequencing with VF2 primer. We used this part in combination with several genes for building our biobricks e.g. BBa_K190061.

••••• maven

Defining efficiency for an RBS seems misleading, since RBS efficiency can only be considered in context of its upstream and downstream sequences as this [http://www.nature.com/nbt/journal/v27/n10/abs/nbt.1568.html article] demonstrated.

••••• iGEM Copenhagen

We attempted to use this BioBrick to assembly a protein expressing device. We were unable to do it with standard assembly but had succesful result utilizing the 2011 [http://2011.igem.org/Team:Copenhagen/Collaboration| DTU-2 user assembly plug'n'play method]

••••• iGEM Amsterdam 2011

Our results show proteins can be expressed at low temperatures using this RBS. From this we conclude that the RBS is useful for translation in cold environments.

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