Generator

Part:BBa_K638403

Designed by: Joseph Harvey   Group: iGEM11_Cambridge   (2011-09-20)
Revision as of 21:10, 21 September 2011 by Marta kkk (Talk | contribs)

Arabinose inducible TorA-Reflectin A1-sfGFP generator

This construct is Reflectin A1 protein with a TorA signal peptide on the N terminus, and superfolder GFP (sfGFP) fused at the C-terminus. The reflectin A1 sequence is codon-optimized for E coli. The TorA signal peptide has been shown to allow export of GFP via the twin arginine pathway. The pBAD promoter is inducible with arabinose.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 1205
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 1144
    Illegal BamHI site found at 1369
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 979
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI site found at 961
    Illegal SapI.rc site found at 2437


[edit]
Categories
Parameters
n/aArabinose inducible TorA-Reflectin A1-sfGFP generator