Generator

Part:BBa_K638103

Designed by: Matthew Jones   Group: iGEM11_Cambridge   (2011-08-04)
Revision as of 20:26, 21 September 2011 by Cat (Talk | contribs)

Arabinose inducible Reflectin 1B generator Produces Reflectin 1B protein on addition of Arabinose.

Usage and Biology

Best used in an E. coli chassis such as strain Bw27783, with constitutive expression of an Arabinose transporter. See our experience of pBAD for some issues with tuning expression levels.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 1205
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 1144
    Illegal BamHI site found at 1260
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 979
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI site found at 961


[edit]
Categories
Parameters
n/aArabinose inducible Reflectin 1B generator