Coding
LigA

Part:BBa_K525710:Design

Designed by: Anna Drong   Group: iGEM11_Bielefeld-Germany   (2011-09-12)
Revision as of 20:21, 21 September 2011 by Adrong (Talk | contribs) (→‎Source)

DNA ligase from Escherichia coli (LigA) with PT7, RBS and His-tag


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 1449
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI.rc site found at 1663


Design Notes

This is the complete sequence of NAD+ -dependent DNA ligase with PT7, RBS and His-tag

Source

PCR on the Escherichia coli TOP 10 DNA cloned into BioBrick Vectors 

   Primers with BioBrick prefix, pT7-Promoter and RBS in the fwd primer. Suffix and His-tag in the rev primer.
       fwd: 5'-acgtgaattcgcggccgcttctagagtaatacgactcactatagggaaagaggagaaaaatggaatcaatcgaacaac-3'
       rev: 5'-acgtctgcagcggccgctactagtaatgatgatgatgatgatggctacccagcaaacgc-3'

References