Part:BBa_K638001:Experience

This experience page is provided so that any user may enter their experience using this part.
Please enter how you used this part and how it worked out.

Applications of BBa_K638001

When extracted and purified, this protein can be used to make optical devices including thin films and diffraction gratings as has been demonstated by [http://www.nature.com/nmat/journal/v6/n7/full/nmat1930.html Kramer et al, 2007.] Additionally, Cambridge iGEM Team 2011 further developed the method for making [http://2011.igem.org/Team:Cambridge/Experiments/Thin_Films thin films].

First the protein must be dissolved in an ionic solvent such as [http://en.wikipedia.org/wiki/Hexafluoro-2-propanol HFIP] (hazardous - use in a fume hood) and then can be coated onto a highly clean smooth substrate. Silicon Wafers and glass microscope slides have been shown to work. A razorblade can be used to scrape across a single drop to form a thin film, but more uniform results can be achieved with the use of a [http://2011.igem.org/Team:Cambridge#/Protocols/Flow_coating flow coater] or a [http://2011.igem.org/Team:Cambridge#/Protocols/Spin_Coating spin coater device].

• 

  Fig.1 Schematic diagram of the flow-coating technique for casting thin films of reflectin protein onto a silicon-wafer substrate. Image taken from [http://www.nature.com/nmat/journal/v6/n7/full/nmat1930.html Kramer et al, 2007.]]]

• 

  Fig.2 Reflectin films exhibiting uniform thicknesses cast from a 15% w/w (left) and 10% w/w (right) reflectin/HFIP solution. Scale bar: 2 cm. Image taken from [http://www.nature.com/nmat/journal/v6/n7/full/nmat1930.html Kramer et al, 2007.] ]]

Compatibility

Has been shown to work with plasmids:

• pSB1A3
• pSB3K3
• pSB1C3

On high copy-plasmids such as pSB1A3, this protein has been shown to go into inclusion bodies.

User Reviews

UNIQ9ea3ff04cfc95447-partinfo-00000001-QINU UNIQ9ea3ff04cfc95447-partinfo-00000002-QINU