Composite

Part:BBa_K546001

Designed by: M. Hesselman   Group: iGEM11_Wageningen_UR   (2011-09-14)
Revision as of 19:03, 21 September 2011 by RubenvanHeck (Talk | contribs)

Lux pL controlled luxR with lux pR controlled AHL degrading enzyme AiiA(lva tag).

This device has two protein generators: The first generator ‘luxpL-RBS-C0062-B0015’ produces an intermediate level of the LuxR protein.

The second generator ‘luxpR-RBS-C0060-B0015’ has a low basal expression of the AiiA enzyme. AiiA is capable of degrading the quorum sensing molecule AHL.

It should be noted that if AHL is added to this system it forms a complex with LuxR which will increase transcription of AiiA while reducing the transcription of LuxR.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 1283
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 1101


[edit]
Categories
//classic/signalling/receiver
//function/cellsignalling
Parameters
control(1) R0063 & (2) R0062
device_typefeedback
directionForward
latencySeconds
protein(1) LuxR & (2) AiiA-LVA
signalling_molecule3OC6HSL (AHL)
switch_point(1) 2nM