Part:BBa_K638202:Experience

Applications of BBa_K638202

The purified his-tagged Reflectin A1 has optical properties, best viewed when made into a thin film. First the protein must be dissolved in an ionic solvent such as HFIP (hazardous - use in a fume hood) and then can be coated onto a highly clean smooth substrate. Silicon Wafers and glass microscope slides have been shown to work, a razorblade can be used to scrape across a single drop to form a thin film, but more uniform results can be achieved with the use of a flow coater or a spin coater device.

The original method for making reflectin thin films was developed by [http://www.nature.com/nmat/journal/v6/n7/full/nmat1930.html Kramer et al.]

Fig.1 Schematic diagram of the flow-coating technique for casting thin films of reflectin protein onto a silicon-wafer substrate.

Fig.2 Reflectin films exhibiting uniform thicknesses cast from a 15% w/w (left) and 10% w/w (right) reflectin/HFIP solution. Scale bar: 2 cm.

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