Intermediate

Part:BBa_K568006:Design

Designed by: Team TU Munich 2011   Group: iGEM11_TU_Munich   (2011-09-14)
Revision as of 18:49, 21 September 2011 by AlexH (Talk | contribs) (→‎Design Notes)

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Intermediate synthetic part of optogenetical AND-Gate


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 33
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 36


Design Notes

The cloning of the part was too difficult due to small fragment sizes. Therefore the whole part was synthesized. It should have originally been cloned from BBa_K228001, BBa_B0015, BBa_K238013 and BBa_J44001.

Source

DNA was synthesized by Geneart.

References