Regulatory

Part:BBa_K549005

Designed by: Sabine Wagner   Group: iGEM11_LMU-Munich   (2011-09-12)
Revision as of 18:16, 21 September 2011 by Jara (Talk | contribs)

LacI promotor fused with the iron dependent regulator fur

The biobrick contains the lacI promotor from E. coli fused with the iron dependent regulator fur from Neisseria meningitidis. Fur regulates genes via binding at fur-binding-boxes. The backbone pSB3C5 is compartible to the standart backbone pSB1C3.

The sequence of fur is missing in the sequence part of this page. fur follows downstream of the RBS with the following sequence: ATGGAAAAATTCAACAATATTGCACAACTGAAAGACAGCGGTCTGAAGGTTACCGGCCCG CGTTTGAAGATTTTGGATTTGTTCGAGACGCATGCGGAAGAGCATTTGAGTGCGGAAGAT GTGTACCGCATTTTGTTGGAAGAGGGTGTGGAAATCGGTGTGGCGACGATTTACCGTGTG CTGACCCAGTTTGAGCAGGCGGGCATTTTGCAACGCCATCATTTTGAAACGGGCAAGGCG GTTTATGAGTTGGACAAAGGCGACCACCATGACCACATCGTCTGCGTGAAGTGCGGCGAG GTAACGGAGTTCCACAATCCCGAAATCGAAGCCCTGCAAGACAAAATCGCGGAAGAAAAC GGCTACCGCATCGTCGATCACGCGCTTTATATGTACGGCGTGTGCAGCGACTGTCAGGCC AAGGGCAAACGTTAA

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI.rc site found at 323


[edit]
Categories
Parameters
None