Coding

Part:BBa_K638001

Designed by: Matthew Jones   Group: iGEM11_Cambridge   (2011-08-04)
Revision as of 16:59, 21 September 2011 by Cat (Talk | contribs)

Reflectin A1 from Loligo

This is the coding region for reflectin protein A1 (originally found in the mantle of squid Loligo pealeii) codon optimised for E. coli. [http://2011.igem.org/Team:Cambridge Cambridge 2011] overexpressed it for purification using the his-tagged generator BBa_K638201 . We also exported it as a TorA fusion.

Usage and Biology

In the squid this protein is believed to self assemble with membranes to form iridophore platelets - stacks of protein ultrastructure evenly spaced to form a Bragg stack. This creates living structural colour. In L. pealeii reflectins are phosphorylated, which changes in phosphorylation status correlating with changes in colours created by thin film interference. [http://2011.igem.org/Team:Cambridge/Project/Background Read more about the background to this protein].

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 22
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
n/aReflectin A1 from Loligo