Composite
ssTorA_CS-

Part:BBa_K627012:Design

Designed by: iGEM11 Potsdam_Bioware   Group: iGEM11_Potsdam_Bioware   (2011-09-21)
Revision as of 16:34, 21 September 2011 by UP Sebastian (Talk | contribs) (→‎References)

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Fusion of TorA sig-seq, TEV protease cleavage site and b-lactamase


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 117
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal XhoI site found at 143
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 796


Design Notes

This biobrick was built by PCR using the following PCR primers:

  • p_TorA-f: CTTCTAGATGAACAATAACGATCTCTTTCAGGCATC
  • o_Bla_igem_r: CTACTAGTATTAACCGGTCCAATGCTTAATCAGTGAGGCAC

Source

TorA signal sequence and the lactamase was amplified via PCR from pJC354, which originates from two iGEM compatible BioBricks: BBa_K208005 and BBa_I757010. The cleavage site was created, based on the infoarmations of the Brenda enzymes database, via 2 oligonucleotides ordered form SigmaAldtrich.

References

ssTorA - https://parts.igem.org/Part:BBa_K208005
blaFL - https://parts.igem.org/Part:BBa_I757010