Composite
ssTorA_CS-

Part:BBa_K627012:Design

Designed by: iGEM11 Potsdam_Bioware   Group: iGEM11_Potsdam_Bioware   (2011-09-21)
Revision as of 16:33, 21 September 2011 by UP Sebastian (Talk | contribs) (→‎Design Notes)

Fusion of TorA sig-seq, TEV protease cleavage site and b-lactamase


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 117
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal XhoI site found at 143
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 796


Design Notes

This biobrick was built by PCR using the following PCR primers:

  • p_TorA-f: CTTCTAGATGAACAATAACGATCTCTTTCAGGCATC
  • o_Bla_igem_r: CTACTAGTATTAACCGGTCCAATGCTTAATCAGTGAGGCAC

Source

References