Signalling

Part:BBa_K549003

Designed by: Sabine Wagner   Group: iGEM11_LMU_Munich   (2011-09-12)
Revision as of 15:37, 21 September 2011 by Jara (Talk | contribs)

Nickel(II) dependent promotor with luxAB as reporter

The biobrick contains the RcnA promotor from E. coli fused with the luxAB reporter gene. E coli strains own the RcnR-regulator gene which direct regulates the transcription of the RcnA nickel cobalt efflux protein from which the promotor is amplified. In presence of nickel the Luciferase is expressed.

The promoter is also a little bit sensitive for Co 2+.



Usage and Biology

This is how the BioBrick is working: Bild-rcnaluxAB.png

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 751
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI site found at 1270


[edit]
Categories
Parameters
None