Part:BBa_K568002

blue light promoter lacZ reporter part

blue light promotor + lacZ

This part is based on the blue light sensor (BBa_K238013) with an additional lacZ Gene. Part (BBa_B0034) was used as RBS.

Usage and Biology

The part provides the possibility to induce lacZ transcription via blue light irradiation.

Blue light leads to dimerisation of YcgF and binding of the repressor YcgE. The formation of the YcgE-YcgF complex leads to the unbinding of YcgE from the YcgZ promoter which activates the transcription of lacZ.

Sequence and Features

Assembly Compatibility:

• 10
  COMPATIBLE WITH RFC[10]
• 12
  COMPATIBLE WITH RFC[12]
• 21
  COMPATIBLE WITH RFC[21]
• 23
  COMPATIBLE WITH RFC[23]
• 25
  COMPATIBLE WITH RFC[25]
• 1000
  COMPATIBLE WITH RFC[1000]

Functional Parameters

n/a	blue light promoter lacZ reporter part

Induction at 465 nm

Experimental Testing