Composite

Part:BBa_K608351

Designed by: Theodhor Tiko   Group: iGEM11_Freiburg   (2011-09-14)
Revision as of 15:14, 21 September 2011 by UsernameV (Talk | contribs) (Usage and Biology)

(correct) temperature sensitive promoter

heat sensitive cI QPI with high promoter, with 6 instead of 8bp between the RBS and cI repressor ATG


Usage and Biology

Freiburg11 Presentation1.jpg

The temperature sensitive promoter functioning at 28-30°C

Freiburg11 Presentation3.jpg The temperature sensitive promoter functioning at 42°C


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 7
    Illegal NheI site found at 30
    Illegal NotI site found at 781
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
//cds/transcriptionalregulator
//cds/transcriptionalregulator/repressor
//function/regulation/transcriptional
//promoter
//regulation/constitutive
//regulation/multiple
Parameters
None