Part:BBa_K608352:Experience

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Please enter how you used this part and how it worked out.

Applications of BBa_K608352

We managed to perform a last-minute, "blind" -ie not knowing if the sequence of the cloning was correct-, and preliminary OD-measurement testing of the part in conjuction with BBa_K608351, as part of our temperature sensitive phage lysis cassette.

We later verified the part sequence. Please refer to our Project Description Page (http://2011.igem.org/Team:Freiburg/Description#The_temperature_sensitive_lysis_cassette) for more Info.

The OD values of the measurement are shown in the chart (please do bear in mind that this was a measurement done under deadline-stress, without the possibility to plan and perform something more concrete before the Wiki-Freeze)

LB with Cm (Chloramphenicol) was used as a blank before every (hourly) measurement

Nr1: Insert is only part BBa_K608351
Nr2: Insert is only this part (BBa K608352)
Nr3: Both parts were correctly assembled (1:BBa_K608351 + 2:BBa K608352)

Therefore BBa_K608352 seem to be functioning properly. More tests are planned if time allows (ie wiki-unfreeze ;) )

User Reviews

UNIQe2f7e17390fee3d5-partinfo-00000000-QINU UNIQe2f7e17390fee3d5-partinfo-00000001-QINU