Part:BBa_K608352
Bacteriophage Lysis Cassette with RBS
Bacteriophage Lysis cassette based on the part BBa_K124017 (note the overlapping coding regions of the sequence in spite of the given sequence for BBa_K124017) with an additional strong RBS (BBa_B0034)
Usage and Biology
The phage lysis cassette consists of the standard bacteriophage λ lysis genes S/S’, R and Rz/Rz1, coded in overlapping sequences with phase 1 and 2 frameshifts.
- S: λ Anti-Holin
- S’: λ Holin
- R: λ Endolysin
- Rz: Putative type-II signal anchor protein
- Rz1: Outer membrane lipoprotein
The phage λ lysozyme (R Endolysin) hydrolyses the beta-1,4-glycosidic bond between N-acetylmuramic acid (MurNAc) and N-acetylglucosamine (GlcNAc), but in order to degrade the cell wall it requires a small transmembrane protein called holin which perforates the membrane for endolysin to gain access to the murein [Ry Young et al]. The auxiliary lysis proteins Rz and Rz1 have long been known to play a vital role but have yet to be assigned a specific function stemming from functional and structural analysis. The assumption is that they form a complex spanning the periplasm and fuse the outer and inner membranes, removing the last physical barrier for cell lysis [Joel Berry et al].
References
Ry Young et al
“Phages will out: strategies of host cell lysis”
Trends in Microbiology Vol 8, Issue 3 (2000) 120-128
Joel Berry et al
“The final step in the phage infection cycle: the Rz and Rz1 lysis proteins link the inner and outer membranes”
Molecular Microbiology 70 (2008) 341–351
Sequence and Features
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
//cds/membrane/lysis
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