Part:BBa_K608352

Bacteriophage Lysis Cassette with RBS

Bacteriophage Lysis cassette based on the part BBa_K124017 (note the overlapping coding regions of the sequence in spite of the given sequence for BBa_K124017) with an additional strong RBS (BBa_B0034)

Usage and Biology

It consists of the standard bacteriophage λ lysis genes, ie S/S’, R, Rz/Rz1, coded in overlapping sequences with phase 1 and 2 frameshifts.

Bacteriophage λ lysis genes at work

• S: λ Anti-Holin
• S’: λ Holin
• R: λ Endolysin
• Rz: Putative type-II signal anchor protein
• Rz1: Outer membrane lipoprotein

The phage λ lysozyme (R Endolysin) hydrolyses the beta-1,4-glycosidic bond between N-acetylmuramic acid (MurNAc) and N-acetylglucosamine (GlcNAc), but in order to degrade the cell wall it requires a small transmembrane protein called holin which perforates the membrane for endolysin to gain access to the murein [Ry Young et al]. The auxiliary lysis proteins Rz and Rz1 have long been known to play a vital role but have yet to be assigned a specific function stemming from functional and structural analysis. The assumption is that they form a complex spanning the periplasm and fuse the outer and inner membranes, removing the last physical barrier for cell lysis [Joel Berry et al].
Sequence and Features

Assembly Compatibility:

• 10
  COMPATIBLE WITH RFC[10]
• 12
  COMPATIBLE WITH RFC[12]
• 21
  COMPATIBLE WITH RFC[21]
• 23
  COMPATIBLE WITH RFC[23]
• 25
  COMPATIBLE WITH RFC[25]
• 1000
  COMPATIBLE WITH RFC[1000]