Translational_Unit

Part:BBa_K515000

Designed by: Atipat Patharagulpong   Group: iGEM11_Imperial_College_London   (2011-09-01)
Revision as of 14:41, 21 September 2011 by RebekkaBauer (Talk | contribs)

IaaM - tryptophan-2-mono-oxygenase IAA tryptophan monooxygenase (IaaM) catalyzes the oxidative carboxylation of L-tryptophan to indole-3-acetamide which acts as an intermediate to the production of indole-3-acetic acid in the IAM pathway. This auxin producing pathway originates from the plant pathogen, Pseudomonas savastanoi

This is one of the parts of our composite auxin expressing construct, BBa_K515100.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 450
    Illegal BamHI site found at 1395
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 157
  • 1000
    COMPATIBLE WITH RFC[1000]


This BioBrick has been sequence verified.

References

[1]Spaepen S. et al., 2007. Indole-3-acetic acid in microbial and microorganism-plant signaling. Federation of European Microbiological Societies Microbiology Reviews , 31, pp.425–448

[edit]
Categories
//cds/enzyme
Parameters
chassisE. coli DH5α
device_typepathway
input_stryptophan
originP. savastanoi
outputIndole acetamide
resistancechloramphenicol