Generator
Part:BBa_I746907:Experience
Designed by: Stefan Milde Group: iGEM07_Cambridge (2008-10-01)
This experience page is provided so that any user may enter their experience using this part.
Please enter
how you used this part and how it worked out.
Applications of BBa_I746907
User Reviews
TU_Munich Team 2011
We tested the part and it worked very well.
our GFP Assay
I746907 (T7 promotor with GFP) in pSB1A2 in BL21DE3 cells
change of medium to M63 (LB shows slight fluorescence itself)
Problem: measurement of Absorbance only every 100 minutes led to spikes in the diagram
results
no concentration dependency of GFP induction as IPTG concentration was in saturation range
for this diagram:
- calculated: Fluorescence/Absorbance
- mean of triplicate measurement
- adjusted to 100 %
- corrected for outliers
- shortened to 700 min
UNIQa04d0a27e540eaab-partinfo-00000000-QINU
UNIQa04d0a27e540eaab-partinfo-00000001-QINU