Coding

Part:BBa_K660201

Designed by: Glasgow Uni 2011   Group: iGEM11_Glasgow   (2011-09-16)
Revision as of 13:17, 21 September 2011 by HannahRalph (Talk | contribs)

Latherin (Surfactant Protein) + 6xHIS Tag

Latherin is a surfactant protein with detergent like properties.

Its normal biological function is temperature regulation and is believed to function by enhancing evaporation from the pelt. It does so by binding to hydrophobic surfaces and allowing them to become wettable.

Part:BBa_K660200

This is a version of the latherin biobrick (K660200) with an attached 6xHIS tag.

Use this biobrick for affinity purification.

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal PstI site found at 691
    Illegal PstI site found at 922
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal PstI site found at 691
    Illegal PstI site found at 922
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 462
    Illegal BglII site found at 1005
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal PstI site found at 691
    Illegal PstI site found at 922
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal PstI site found at 691
    Illegal PstI site found at 922
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
None