Composite

Part:BBa_K551000

Designed by: Laurence Van Melderen   Group: iGEM11_ULB-Brussels   (2011-09-19)
Revision as of 13:08, 21 September 2011 by ULB (Talk | contribs)

FRT-CM-FRT

This part is composed of two mutated Flipase Recognition Targets (FRT') flanking the Chloramphenicol resistance gene. The mutation removes the EcoRI restriction site from the canonical FRT sequence. It is the same as BBa_J61025 but without the scar between the first FRT' and CmR.

The two FRT' sequences allow the excision of the flanked sequence by the Flipase recombinase.

It's been tested with success for resistance to Chloramphenicol and excision per the pINDEL plasmid.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
//function/recombination/flp
Parameters
resistance20 g/ml