Composite

Part:BBa_K551000:Design

Designed by: Laurence Van Melderen   Group: iGEM11_ULB-Brussels   (2011-09-19)
Revision as of 12:57, 21 September 2011 by ULB (Talk | contribs) (→‎Design Notes)

FRT-CM-FRT


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

The initial part (BBa_J61025) wasn't working, so we remade it from the intermediate CmR-FRT (BBa_J61024). For that we made a PCR with a primers containing the FRT sequence and the RFC10 prefix; this means there is no scar between the first FRT and CmR.

Source

It comes from PCR of a registry part

References