Composite

Part:BBa_K568003

Designed by: Team TU Munich 2011   Group: iGEM11_TU_Munich   (2011-09-14)
Revision as of 12:27, 21 September 2011 by AlexH (Talk | contribs)

T7 promoter lacZ reporter part

beta-galactosidase expression upon T7 polymerase binding


Usage and Biology

This part can be used as a reporter plasmid. It expresses beta-galactosidase if there is a T7 polymerase (or a mutant variant) nearby or transcribed through another part. The polymerase binds to the T7 promoter and transcription of the lacZ-Gene can occur, leading to beta-galactosidase expression.


Experimental Testing

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
None