Plasmid

Part:BBa_K541800

Designed by: Mustafa Elitok   Group: iGEM11_Fatih_Turkey   (2011-09-21)
Revision as of 11:52, 21 September 2011 by Registry (Talk | contribs)

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B.subtilis and E.coli Episomal Shuttle Vector with Consitutive RFP to express in E.coli

This episomal vector has within its BioBrick region a constitutively expressed RFP gene in E.coli. It uses LacI promoter, B0034, mRFP1 (E0010) and B0015.

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal prefix found in sequence at 5187
    Illegal suffix found in sequence at 1
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 5187
    Illegal SpeI site found at 2
    Illegal PstI site found at 16
    Illegal NotI site found at 9
    Illegal NotI site found at 5193
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 5187
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal prefix found in sequence at 5187
    Illegal suffix found in sequence at 2
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal prefix found in sequence at 5187
    Illegal XbaI site found at 5202
    Illegal SpeI site found at 2
    Illegal PstI site found at 16
    Illegal AgeI site found at 5997
    Illegal AgeI site found at 6109
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 1533
    Illegal BsaI.rc site found at 4261
    Illegal SapI site found at 3178


[edit]
Categories
//chassis/prokaryote/bsubtilis
//chassis/prokaryote/ecoli
Parameters
None