Plasmid_Backbone
K592200

Part:BBa_K592200

Designed by: Erik Gullberg, Antonio Ascue Avalos, Erik Lundin   Group: iGEM11_Uppsala-Sweden   (2011-09-21)
Revision as of 11:29, 21 September 2011 by Antonio (Talk | contribs)

Low to medium copy BioBrick standard vector

BBa_K592200 is a BioBrick standard vector with low to medium copy p15A replication origin (BBa_I50032) and spectinomycin antibiotic resistance marker, usable for Lambda Red recombineering in E coli. The E coli His operon terminator BBa_B0053 has been replaced with the late terminator of the Salmonella phage P22.


K592200-J04450.png

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Plasmid lacks a prefix.
    Plasmid lacks a suffix.
    Illegal EcoRI site found at 3116
    Illegal NheI site found at 1403
    Illegal SpeI site found at 2
    Illegal PstI site found at 16
    Illegal NotI site found at 9
    Illegal NotI site found at 3122
  • 21
    INCOMPATIBLE WITH RFC[21]
    Plasmid lacks a prefix.
    Plasmid lacks a suffix.
    Illegal EcoRI site found at 3116
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal prefix found at 3116
    Illegal suffix found at 2
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal prefix found at 3116
    Plasmid lacks a suffix.
    Illegal XbaI site found at 3131
    Illegal SpeI site found at 2
    Illegal PstI site found at 16
    Illegal NgoMIV site found at 1969
    Illegal AgeI site found at 994
    Illegal AgeI site found at 1317
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Plasmid lacks a prefix.
    Plasmid lacks a suffix.
    Illegal BsaI site found at 289


[edit]
Categories
//dna/chromosomalintegration
//plasmidbackbone/assembly
//plasmidbackbone/copynumber/medium
//plasmidbackbone/operation
Parameters
chassis
copies10-12
functionLambda Red recombineering
mcsBioBrick
originp15A
resistanceS