Generator
pT7 GFP T7

Part:BBa_K606035

Designed by: Axel Séguret   Group: iGEM11_Paris_Bettencourt   (2011-09-18)
Revision as of 10:28, 21 September 2011 by Cyrpaut (Talk | contribs)

pT7 SpoVG GFP T7terminator.

T7 RNA polymease controlled GFP expression system for B. subtilis and E. coli

Usage and Biology

This part is the concatenation of the promoter pT7 (I719004) with the RBS SpoVG (K143021), that is design for B. subtilis but that also works in E. coli. This part works very efficiently and have been caracterized in E. coli BL21 cells. The part is in pSB1C3.

See the experiment page of this part or our wiki page[http://2011.igem.org/Team:Paris_Bettencourt/Experiments/T7_diffusion] for additional information

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 917
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 693


Characterization

Growth of this part in BL21 strain under various concentration of IPTG.

Fig1: Growth curves for BL21 strain carrying the part, in the presence of cloramphenicol

See the experiment page for more details.

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Categories
Parameters
None