Composite

Part:BBa_K546001:Experience

Designed by: M. Hesselman   Group: iGEM11_Wageningen_UR   (2011-09-14)
Revision as of 09:56, 21 September 2011 by Youri (Talk | contribs) (→‎User Reviews)

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Applications of BBa_K546001

User Reviews

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Protocol

Functionality of this part was tested by treating AHL mixtures with strains expressing this part. Liquid cultures were grown overnight, 1 mL aliquots of these cultures were spun down and resuspended in varying concentrations of AHL in PBS (to start the AHL degradation reaction). This was also done with a culture that contains BBa_K546002 (where aiiA is replaced with GFP).

After a 3h incubation, the supernatant was collected and subjected to a reporter strain: BBa_K546002. 200 µL samples of reporter strain/AHL mixtures were analyzed with a Molecular Devices Spectramax M2 spectrophotometer, detecting GFP. The result is shown below.


AHL degradation by AiiA reported by BBa K546002correct.png

This part is functional, as AHL concentrations drop after incubation with E. coli transformed with this part. The GFP expression is much higher in the non treated mixtures, namely.

Team Wageningen_UR 2011

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UNIQ26a879ceb8400ee9-partinfo-00000002-QINU