Coding

Part:BBa_K539711:Design

Designed by: Yang, Yi-Pei   Group: iGEM11_NCTU_Formosa   (2011-09-20)
Revision as of 03:06, 21 September 2011 by Registry (Talk | contribs)

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ilvC (ketol-acid reductoisomerase NAD(P)-binding)


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 592
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 178
    Illegal BsaI.rc site found at 997


Design Notes

Because of the four RE site, EcoR1, Xba1, Spe1 and Pst1, before and after the coding gene, we have to delete those RE site in kivd. The RE site in the part has been modified by point mutation.


Source

It comes from Escherichia coli str. K-12 substr. MG1655


References