Coding

Part:BBa_K539714:Design

Designed by: Yang, Yi-Pei   Group: iGEM11_NCTU_Formosa   (2011-09-20)
Revision as of 02:35, 21 September 2011 by Registry (Talk | contribs)

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kivd (alpha-ketoisovalerate decarboxylase)


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 578
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

In order to design the four RE site, EcoR1, Xba1, Spe1 and Pst1, before and after the coding region.However, there is one RE site, EcoR1, in kivd. The RE site in kivd has been modified by point mutation.



Source

It is cloned form Lactococcus lactis subsp. lactis KF147

References