Coding
LuxC

Part:BBa_K590059:Design

Designed by: Chris Choe and David Zong   Group: iGEM11_Washington   (2011-09-15)
Revision as of 23:51, 20 September 2011 by Anjuhye (Talk | contribs) (→‎Design Notes)

LuxC; standalone, E. Coli codon optimized


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

Lux C, Lux D and Lux E were re-optimized to have cloning-friendly GC content. Primers were designed to synthesize the genes separately each in their own PCR reaction. Another PCR was used to amplify the respective pieces, and each piece was ligated into psb1C3. Once each gene was in psb1C3, it was re-amplified out and ligated into a vector containing ADC. This is done step wise until the full construct is obtained.

Source

Gene-synthesis

References