Coding

Part:BBa_K590018:Design

Designed by: Michael Brasino, Alicia Wong, Rashmi Ravichandran   Group: iGEM11_Washington   (2011-09-15)
Revision as of 22:48, 20 September 2011 by Saushun (Talk | contribs) (→‎Source)

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mamMNOPA in vector pGA3K3


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 288
    Illegal PstI site found at 74
    Illegal PstI site found at 4039
    Illegal PstI site found at 4757
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 288
    Illegal NheI site found at 1331
    Illegal PstI site found at 74
    Illegal PstI site found at 4039
    Illegal PstI site found at 4757
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 288
    Illegal BglII site found at 361
    Illegal BamHI site found at 1628
    Illegal BamHI site found at 2148
    Illegal BamHI site found at 4144
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 288
    Illegal PstI site found at 74
    Illegal PstI site found at 4039
    Illegal PstI site found at 4757
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 288
    Illegal PstI site found at 74
    Illegal PstI site found at 4039
    Illegal PstI site found at 4757
    Illegal NgoMIV site found at 3180
    Illegal NgoMIV site found at 3553
    Illegal NgoMIV site found at 3709
    Illegal NgoMIV site found at 3832
    Illegal NgoMIV site found at 4627
    Illegal NgoMIV site found at 4814
    Illegal NgoMIV site found at 5851
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 477
    Illegal BsaI.rc site found at 2431
    Illegal SapI.rc site found at 56


Design Notes

Partial sequence confirmed


Source

Made by Gibson Cloning Method.

References