Measurement
ChiPm-lacZ

Part:BBa_K564013

Designed by: Anna Lewinska   Group: iGEM11_DTU-Denmark   (2011-09-20)
Revision as of 22:41, 20 September 2011 by Alewinska88 (Talk | contribs)

Upstream mutated chitoporin part fused with lacZ

ChiP encodes enterobacterial chitoporin required for uptake of chitin-derived oligosaccharides. This outer membrane porin is required for Salmonella and E.coli growth on chitooligosaccharides as a sole source of both carbon and nitrogen. It is only synthesized in the presence of these substrates. In nature it is regulated by sRNAs. In this biobrick we altered the binding site for ChiP regulator – ChiX. It was done in order to show that the regulatory system depends on the specific base pairing between ChiP and its regulator. The upstream part of this gene is fused with reporter gene lacZ to allow checking if the regulation works correctly.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


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Categories
Parameters
None