Measurement
ChiPm-lacZ
Part:BBa_K564013
Designed by: Anna Lewinska Group: iGEM11_DTU-Denmark (2011-09-20)
Revision as of 22:35, 20 September 2011 by Alewinska88 (Talk | contribs) (New page: _NOTOC__ <partinfo>BBa_K564015 short</partinfo> ChiP encodes enterobacterial chitoporin required for uptake of chitin-derived oligosaccharides. This outer membrane porin is required for S...)
_NOTOC__ ChiP promoter and 5' UTR; RBS mutated AAGAGG-->AGGAGA
ChiP encodes enterobacterial chitoporin required for uptake of chitin-derived oligosaccharides. This outer membrane porin is required for Salmonella and E.coli growth on chitooligosaccharides as a sole source of both carbon and nitrogen. It is only synthesized in the presence of these substrates. In nature it is regulated by sRNAs. In this biobrick we altered the binding site for ChiP regulator – ChiX. It was done in order to show that the regulatory system depends on the specific base pairing between ChiP and its regulator. The upstream part of this gene is fused with reporter gene lacZ to allow checking if the regulation works correctly.
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
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Categories
Parameters
| None |