Composite

Part:BBa_K568000

Designed by: Team TU Munich 2011   Group: iGEM11_TU_Munich   (2011-09-01)
Revision as of 22:23, 20 September 2011 by Sue (Talk | contribs)

Red light sensor

Single red light sensor without additional genes. This part can pe clonend anywhere one wants to.


Usage and Biology

Red light induces the autophosphorylation at the cytosolic site of cph8. This leads to phosphorylation of OmpR which subsequently binds to OmpC promotor and enables transcription of a following gene.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 7
    Illegal NheI site found at 30
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 644
    Illegal XhoI site found at 1786
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 971
  • 1000
    COMPATIBLE WITH RFC[1000]


Functional Parameters

n/aRed light sensor

Induction with red light at 705 nm

Since sensor is constitutive switched on, red light signalling can be shut down with 650 nm.

[edit]
Categories
Parameters
n/aRed light sensor