Not Released
Sample In stock
Experience: Works
Not Used
Get This Part
Coding

Part:BBa_K613016:Design

Designed by: Lilia Salimova   Group: iGEM11_EPF-Lausanne   (2011-09-20)
Revision as of 22:15, 20 September 2011 by Registry (Talk | contribs)

(diff) ← Older revision | Latest revision (diff) | Newer revision → (diff)


TetR P39K mutant


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

The mutation was introduced by site-directed mutagenesis


Source

The TetR gene was PCR-amplified from the Repressilator plasmid. We then introduced the V36F mutation by site-directed mutagenesis. This mutation had already been described by Krueger et al (2007).

References