Coding

Part:BBa_K553000:Design

Designed by: Giulio Bernardinelli, Veronica Parisi, Francesca Cesaratto   Group: iGEM11_UNITS_Trieste   (2011-09-17)
Revision as of 21:47, 20 September 2011 by Giulio (Talk | contribs) (→‎References)

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TraR trans-activator


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

the part was generated by using the following primers:


TraR fw

5' GTTTCTTCGAATTCGCGGCCGCTTCTAGATCGACGACTGGCTGGACAAGC 3'

TraR rev

5' GTTTCTTCCTGCAGCGGCCGCTACTAGTATCAGATGAGTTTCCGCCGGATG 3'


pcr protocol:

95° 5' | 10x (93° 30" | 56° 30" | 72° 40") | 23x ( (93° 30" | 65° 30" | 72° 40") | 72° 7' | 4° ∞


The part contains also the BBa_B0034 RBS and BBa_B0015 double terminator.

Source

The gene was extracted by pcr from Agrobacterium tumefaciens gDNA.

http://www.ncbi.nlm.nih.gov/gene/1224220

The sequencing revealed mutations in our strain that do not affect the part functionality.

References

Piper KR, Beck von Bodman S, Far- rand SK. 1993. Conjugation factor of Agrobacterium tumefaciens regulates Ti plasmid transfer by autoinduction. Nature 362:448–50

Zhang L, Murphy PJ, Kerr A, Tate ME. 1993. Agrobacterium conjugation and gene regulation by N-acyl-L-homoserine lactones. Nature 362:446–48