Signalling

Part:BBa_K608101

Designed by: Julia Müller   Group: iGEM11_Freiburg   (2011-09-14)
Revision as of 20:46, 20 September 2011 by Juliimapril (Talk | contribs)

CcaR, green light response regulator


CcaR is the response regulator to the green light receptor CcaS.
It belongs to the family of OmpR regulator class. CcaR consists of an N-terminal receiver domain that can be
phosphorylated by CcaS and a C-terminal DNA-binding domain that binds directly to the promoter region of cpcG2.

Ccar.jpg

Usage and Biology

when we amplified CcaR from the whole Synechocystis genome via PCR,
the primer had an overhang which also has a Ribosome Binding Site.
If you want to use it, you can clone it behind a promotor of choice.


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 380
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
None