Plasmid
pJEJAM1
Part:BBa_K678049
Designed by: DTU-Denmark-2 Group: iGEM11_DTU-Denmark-2 (2011-09-13)
pJEJAM1
pJEJAM1 is a plasmid intended for transient transfection of mammalian cells. pJEJAM1 consists of the strong constitutive cytomegalovirus (CMV) promoter, the green fluorescence protein (GFP), the BGK polyA and the hygR marker cassette used for selection, an ori for propagation in E.coli and and ampR resistance cassette (Figure below).
Sequence and Features
Assembly Compatibility:
- 10INCOMPATIBLE WITH RFC[10]Illegal EcoRI site found at 2216
Illegal PstI site found at 2306 - 12INCOMPATIBLE WITH RFC[12]Illegal EcoRI site found at 2216
Illegal PstI site found at 2306 - 21INCOMPATIBLE WITH RFC[21]Illegal EcoRI site found at 2216
- 23INCOMPATIBLE WITH RFC[23]Illegal EcoRI site found at 2216
Illegal PstI site found at 2306 - 25INCOMPATIBLE WITH RFC[25]Illegal EcoRI site found at 2216
Illegal PstI site found at 2306
Illegal NgoMIV site found at 3071 - 1000COMPATIBLE WITH RFC[1000]
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Categories
Parameters
//chassis/eukaryote
//chassis/eukaryote/human
//direction/forward
//plasmid/expression
//chassis/eukaryote/human
//direction/forward
//plasmid/expression
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