Regulatory
PT5lac

Part:BBa_K592008

Designed by: Erik Lundin   Group: iGEM11_Uppsala-Sweden   (2011-09-11)
Revision as of 18:19, 20 September 2011 by Erlu2699 (Talk | contribs)

T5-lac Promoter

T5-lac promoter is a hybrid promoter made from the phage T5 early promoter and the lac-operon. It contains three LacI binding sites and remains repressed in LacIq strains where LacI is expressed in high levels. It is inducible by IPTG, and recognizable by E coli RNA polymerase. Compared to the T7 promoters, T5-lac promoter doesn't require the co-expression of phage polymerase.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
//promoter
Parameters
None