Coding
cjBlue

Part:BBa_K592011

Designed by: Antonio Ascue Avalos   Group: iGEM11_Uppsala-Sweden   (2011-09-18)
Revision as of 18:08, 20 September 2011 by Erlu2699 (Talk | contribs)

cjBlue, green chromoprotein

This chromoprotein from the Cnidopus japonicus sea anemone, cjBlue, naturally exhibits dark green color when expressed. Compared to amilCP(BBa_K592009) and amilGFP(BBa_K592010), the color development is slower. On agar plates and in liquid culture, the color is readily visible to naked eye after 24-48 hours of incubation. The DNA was codon optimized for expression in E coli and synthesized by the Korean company Bioneer Corporation.

Usage and Biology

This part is useful as a reporter.

CjBlue plate small.jpg

Escherichia coli constitutively expressing cjBlue (BBa_K592011).


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
//cds/reporter/chromoprotein
//collections/chromoprotein/uppsala
//function/reporter/pigment
Parameters
colorBlue
protein