Composite

Part:BBa_K515106

Designed by: Atipat Patharagulpong   Group: iGEM11_Imperial_College_London   (2011-09-07)
Revision as of 11:22, 20 September 2011 by Cs3109 (Talk | contribs)

J23103 promoer - RBS B0034-RFP E1010 - Holin K112805 - endolysin K112806

A composite part comprising of a J23100 promotor, holin (BBa_K112805) and endolysin (BBa_K112806). This part has been to designed to lyse any cell which does not contain part BBa_K515104 in the genome.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 7
    Illegal NheI site found at 30
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 616
    Illegal AgeI site found at 728
    Illegal AgeI site found at 1730
    Illegal AgeI site found at 1800
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
//biosafety/kill_switch
//function/celldeath
Parameters
chassisE. coli DH5α
controlJ23103
device_typeLysis component of containment module
originP. aeruginosa
resistanceChloramphenicol