Measurement
PchiP-lacZ
Part:BBa_K564012
Designed by: Anna Lewinska Group: iGEM11_DTU-Denmark (2011-09-20)
promoter and RBS of E. coli chitoporin translationally fused with lacZ.
ChiP encodes enterobacterial chitoporin required for uptake of chitin-derived oligosaccharides. This outer membrane porin is required for Salmonella and E.coli growth on chitooligosaccharides as a sole source of both carbon and nitrogen. It is only synthesized in the presence of these substrates. In nature it is regulated by sRNAs.The upstream part of this gene is fused with reporter gene lacZ to allow checking if the regulation works correctly.
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
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Categories
Parameters
None |