Coding
mdnED

Part:BBa_K627000:Design

Designed by: iGEM2011 Potsdam_Bioware   Group: iGEM11_Potsdam_Bioware   (2011-09-20)
Revision as of 08:36, 20 September 2011 by Registry (Talk | contribs)

(diff) ← Older revision | Latest revision (diff) | Newer revision → (diff)


ABC transporter and N-acetyltransferase from mdn-cluster


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 281
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI.rc site found at 777
    Illegal SapI.rc site found at 1098


Design Notes

This biobrick was built by PCR using the following PCR primers Forward primer: TAAATGAATTCGCGGCCGCTTCTAGATGCCTCAATATACTACTAAAC Reverse primer: ATTTCTGCAGCGGCCGCTACTAGTATCAGCAAACCCTACTTAATTTC To insert mdnED in the vector pSb1C3, the resulting PCR product and the vector were digested with the restriction enzymes, EcoRI and SpeI.


Source

The BioBrick mdnDE as a part of the microviridin gene (mdn) cluster was isolated from Microcystis aeruginosa strain NIES-843.

References