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iccdB1.0

Part:BBa_K658001:Design

Designed by: Yidan Hu   Group: iGEM11_XMU-China   (2011-09-20)
Revision as of 07:53, 20 September 2011 by Registry (Talk | contribs)

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a bacteria population-control device with RBS1.0 driven by lacl+pL


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 718
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 2344
    Illegal BsaI.rc site found at 1874


Design Notes

The bacteria population-control system was once a two-plasmid system. In order to enhance its stability and efficiency, we integrated target sequences into one plasmid.


Source

K176003

References