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Part:BBa_K510022

Designed by: David Caballero   Group: iGEM11_UPO-Sevilla   (2011-09-19)
Revision as of 22:16, 19 September 2011 by Dcabpra (Talk | contribs)

attTn7


TnsABC+D transposase machinery causes the site-specific insertion of the Tn7 transposon in the attTn7 (from attachment site) sequence, which is found in many bacteria. This insertion site is well conserved in Gram-negative bacteria, an even in higher organisms until humans. However, here we add a portable attTn7 to allow using the [http://2011.igem.org/Team:UPO-Sevilla/Foundational_Advances/MiniTn7/Overview miniTn7 BioBrick toolkit] in any organism as well as in plasmids. All the derivatives of the miniTn7 BioBrick toolkit could be inserted in the attTn7 site by transposition.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


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Categories
Parameters
None