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Part:BBa_K510022:Design

Designed by: David Caballero   Group: iGEM11_UPO-Sevilla   (2011-09-19)
Revision as of 22:12, 19 September 2011 by Registry (Talk | contribs)

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attTn7


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

The attTn7 site was flanked by prefix and suffix for cloning purposes.


Source

The attTn7 was obtained from the purified genome of E. coli MC4100 by PCR (using a high accuracy Pfu polymerase) with these primers: gaattcgcggccgcttctagagTGCAGCTGCTGGCTTACCATG and ctgcagcggccgctactagtaACATGGAGTTGGCAGGATGTTTG.

References