Part:BBa_K510021

pUC18R6KT-miniTn7BB-Gm-Lux

The mini-Tn7-Gm-Lux artificial transposon has been constructed based on pUC18R6KT-miniTn7-Gm (BBa_K510012) by inserting a pBad/Lux operon (BBa_K325909) in its BioBrick cloning site (BCS). This plasmid can be used for visualization purposes or to brand a strain because of its insertion in the genome of the working organism and split the antibiotic resistance cassette with the temporary expression of Flp recombinase.

Sequence and Features

Assembly Compatibility:

• 10
  INCOMPATIBLE WITH RFC[10]

  Illegal prefix found in sequence at 4528
  Illegal suffix found in sequence at 1
  
• 12
  INCOMPATIBLE WITH RFC[12]

  Illegal EcoRI site found at 4528
  Illegal NheI site found at 5762
  Illegal NheI site found at 8728
  Illegal SpeI site found at 2
  Illegal PstI site found at 16
  Illegal NotI site found at 9
  Illegal NotI site found at 4534
  
• 21
  INCOMPATIBLE WITH RFC[21]

  Illegal EcoRI site found at 4528
  Illegal BglII site found at 3212
  Illegal BglII site found at 3483
  Illegal BglII site found at 3769
  Illegal BglII site found at 7726
  Illegal BamHI site found at 5701
  Illegal XhoI site found at 8556
  
• 23
  INCOMPATIBLE WITH RFC[23]

  Illegal prefix found in sequence at 4528
  Illegal suffix found in sequence at 2
  
• 25
  INCOMPATIBLE WITH RFC[25]

  Illegal prefix found in sequence at 4528
  Illegal XbaI site found at 4543
  Illegal SpeI site found at 2
  Illegal PstI site found at 16
  Illegal AgeI site found at 5536
  
• 1000
  INCOMPATIBLE WITH RFC[1000]

  Illegal BsaI site found at 10115
  Illegal BsaI.rc site found at 1854
  Illegal BsaI.rc site found at 7124
  Illegal SapI site found at 518
  Illegal SapI site found at 5518
  Illegal SapI.rc site found at 10440