Device
Part:BBa_K510019
Designed by: Victor Alvarez, Felix Reyes and Yolanda Gonzalez Group: iGEM11_UPO-Sevilla (2011-09-19)
Improved Flip Flop (Module I)
Here we show the final structure of our improved bistable. As well as the two operons with their protein fusions and the promoter with the asRNA, we show the different restriction sites we have introduced and the enzymes that target each site. Finally, the total length of our construction is indicated in the figure.
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12INCOMPATIBLE WITH RFC[12]Illegal NheI site found at 3130
- 21INCOMPATIBLE WITH RFC[21]Illegal BamHI site found at 940
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal AgeI site found at 4392
Illegal AgeI site found at 4504 - 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI site found at 271
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Categories
Parameters
None |